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How fluorescence works

Jablonski diagrams

https://alluxa.com/optical-filter-applications/fluorescence-filters-microscopy-imaging/

Spectra

Resolution

Lateral resolution

Axial resolution

https://andor.oxinst.com/learning/view/article/microscopy-dictionary-a-to-z-glossary

Light-microscopy modalities

Widefield versus confocal

Ishikawa-Ankerhold, H.C.; Ankerhold, R.; Drummen, G.P.C.

Advanced Fluorescence Microscopy Techniques—FRAP, FLIP, FLAP, FRET and FLIM. Molecules 2012, 17, 4047-4132.

Transmitted ligh: brightfield

Confocal versus 2-photon

Digital image

https://hamamatsu.magnet.fsu.edu/articles/digitalimagebasics.html

Impact of acquisition parameters on quantitative measurements

Culley, S., Caballero, A. C., Burden, J. J., & Uhlmann, V. (2024). Made to measure: An introduction to quantifying microscopy data in the life sciences. Journal of Microscopy, 295, 61–82.

Quantitative bioimaging experiment workflow

Senft RA, Diaz-Rohrer B, Colarusso P, et al. A biologist's guide to planning and performing quantitative bioimaging experiments. Plos Biology. 2023 Jun;21(6):e3002167.

Centre for Cell & Molecular Dynamics

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